Review

vero e6 tmprss2 (ATCC)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC vero e6 tmprss2

Vero E6 Tmprss2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 18116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vero+e6/Vero/pmc13651819-173-0-2
Average 99 stars, based on 18116 article reviews

vero e6 tmprss2 - by Bioz Stars, 2026-10

99/100 stars

Images

1) Product Images from "Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses"

Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses

Journal: iScience

doi: 10.1016/j.isci.2026.117639

Figure Legend Snippet:

Techniques Used: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software

Expressing:

Article Title: Functional divergence of the Bhanja virus NSs protein modulates interferon antagonism and viral fitness across divergent lineages
Article Snippet: Human cells: A549 (lung epithelial) [ ], A549-NPro (expressing BVDV NPro; IRF-3 degradation) [ , ], A549-VNPro (generated by simultaneously and constitutively expressing PIV5-V and BVDV NPro; STAT1 and IRF-3 degradation) [ ], Huh7.5-T7, Huh7 Lunet-T7, Huh7-ViperinKD (hepatocellular carcinoma; T7 polymerase-expressing or Viperin-knockdown variants) [ , ], SK-N-SH (neuroblastoma) [ ], DAOY (medulloblastoma) [ ], U373-MG (glioblastoma) [ ], SVG p12 (astroglia, ATCC CRL-8621) [ ]. .. Animal-derived cells: Vero E6 (African green monkey kidney) [ ], BHK-21 (hamster kidney) [ ], BSR-T7/5 (BSR expressing T7 polymerase) [ ], MDBK (bovine kidney, ATCC CCL-22) [ ], MDCK (canine kidney, ATCC CCL-34) [ ], RAW264.7 (mouse monocyte/macrophage, ATCC TIB-71), MRK101 (vole kidney) [ ], QT35 (Japanese quail fibrosarcoma, ECACC 93120832) [ ], DF-1 (chicken fibroblast, ATCC CRL-12203) [ ], E. Derm (horse fibroblast, ATCC CCL-57) [ ]. .. Media: DMEM (ThermoFisher) with 10% heat-inactivated foetal bovine serum (FBS) for A549, Vero E6, BHK-21, BSR variants, and MDBK.

Cell Culture:

Article Title: RNF138-Mediated Ubiquitination and Degradation of NS5 Restricts Tick-Borne Encephalitis Virus Infection.
Article Snippet: .. .2 Cell Culture, Viruses, and Titrations EK293T (CRL-11268), A549 (CCL-185), Vero-E6 (CRL-1586), and eLa (CRM-CCL-2) cells were obtained from the American ype Culture Collection (ATCC, Manassas, VA, USA). ..

Article Title: Porcine epidemic diarrhea virus suppresses DHX9-mediated antiviral transcription through its nucleocapsid protein.
Article Snippet: .. Vero E6 and 293T cells were purchased from American Type Culture Collection (Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific, 11995500) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, 10099). .. IPECJ2 cells (porcine small intestinal epithelial cell line, DSMZ, Germany) were cultured in RPMI 1640 medium (Thermo Fisher Scientific, 11875119) supplemented with 10% FBS.

Transfection:

Article Title: Laboratory adaptation of the SARS-CoV-2 Delta variant in Vero E6 cells enhances in vitro growth but attenuates human TMPRSS2-dependent fitness, pathogenicity, and transmission
Article Snippet: .. Cells, transfection, protein purification, and SPR TMThe following cell lines were used: Vero E6 (ATCC CRL-1586 ), Vero E6hTMPRSS2+ (JCRB 1819), LLC-MK2 (ATCC CCL-7TM ), Huh-7 (JCRB 0403), Caco-2 (ATCC HTB-37TM ), HEK-293 T (ATCC CRL3216TM ), and HEK-293F (Thermo Fisher Scientific, Cat. No. R79007). .. HeLahACE2+ cells, a derivative cell line stably expressing human ACE2 generated from HeLa cells (ATCC CCL-2TM).

Protein Purification:

SPR Assay:

Modification:

Article Title: Quantitative Tissue Proteomics Reveals Protein Signatures Associated with SARS-CoV-2 Variant Infection in Hamsters.
Article Snippet: Since its emergence in 2019, circulating SARS-CoV-2 has been dominated by waves of genetically distinct variants with varying pathogenicity.. Understanding the multidimensional responses to SARS-CoV-2 infection and their associations with pathogenesis is critical for developing therapies to prevent severe illness and death.. Here, we applied quantitative proteome and phosphoproteome analyses to compare host responses to infections with an ancestral variant (WA-1/2020), a Delta variant (B.1.617.2), and an Omicron variant (BA.1) of SARS-CoV-2 in Syrian golden hamster tissues at 5 days postinfection, when peak inflammatory responses were observed.

Image Search Results

Journal: iScience

Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses

doi: 10.1016/j.isci.2026.117639

Figure Lengend Snippet:

Article Snippet: VERO-E6-TMPRSS2 , ATCC , CCL-1587.

Techniques: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software

(A) Representative immunofocus assay images showing viral foci produced by parental wtIG690 and recombinant rIG690 following infection of Vero E6 cell monolayers. (B) Replication kinetics of wtIG690 and rIG690 in A549 and A549-VNPro cells. Cell monolayers were infected at an MOI of 0.1 FFU/cell and culture supernatants harvested at the indicated times p.i. for titration by focus-forming assay on Vero E6 cells. Viral titres are presented as Log 10 FFU/ml. Data represent the mean ± SD from three biological replicates. (C,D) Representative analysis of viral protein expression during wtIG690 and rIG690 infection of A549 (C) and A549-VNPro (D) cells. Cell lysates collected at the indicated times p.i. from growth curves described in (B) were analysed by western blotting for expression of BHAV N and NSs proteins. Tubulin was used as a loading control. Representative blots from the three independent experiments are shown.

Journal: PLOS Pathogens

Article Title: Functional divergence of the Bhanja virus NSs protein modulates interferon antagonism and viral fitness across divergent lineages

doi: 10.1371/journal.ppat.1014645

Figure Lengend Snippet: (A) Representative immunofocus assay images showing viral foci produced by parental wtIG690 and recombinant rIG690 following infection of Vero E6 cell monolayers. (B) Replication kinetics of wtIG690 and rIG690 in A549 and A549-VNPro cells. Cell monolayers were infected at an MOI of 0.1 FFU/cell and culture supernatants harvested at the indicated times p.i. for titration by focus-forming assay on Vero E6 cells. Viral titres are presented as Log 10 FFU/ml. Data represent the mean ± SD from three biological replicates. (C,D) Representative analysis of viral protein expression during wtIG690 and rIG690 infection of A549 (C) and A549-VNPro (D) cells. Cell lysates collected at the indicated times p.i. from growth curves described in (B) were analysed by western blotting for expression of BHAV N and NSs proteins. Tubulin was used as a loading control. Representative blots from the three independent experiments are shown.

Article Snippet: Animal-derived cells: Vero E6 (African green monkey kidney) [ ], BHK-21 (hamster kidney) [ ], BSR-T7/5 (BSR expressing T7 polymerase) [ ], MDBK (bovine kidney, ATCC CCL-22) [ ], MDCK (canine kidney, ATCC CCL-34) [ ], RAW264.7 (mouse monocyte/macrophage, ATCC TIB-71), MRK101 (vole kidney) [ ], QT35 (Japanese quail fibrosarcoma, ECACC 93120832) [ ], DF-1 (chicken fibroblast, ATCC CRL-12203) [ ], E. Derm (horse fibroblast, ATCC CCL-57) [ ].

Techniques: Produced, Recombinant, Infection, Titration, Focus Forming Assay, Analysis, Expressing, Western Blot, Control

(A) Replication of wtIG690 in a panel of vertebrate cell lines derived from different host species and tissues. Cell monolayers were infected with wtIG690 at an MOI of 0.01 FFU/cell (light blue) or 0.1 FFU/cell (dark blue). At 5 d p.i., culture supernatants were harvested and viral titres determined by focus-forming assay on Vero E6 cells. Viral titres are presented as Log 10 FFU/ml. M, monkey; Mo, mouse; V, vole; Ch, chicken; Qu, quail, Ho, horse; WBC, white blood cells; K, kidney; E, embryo; S, skin. Experiments were performed in duplicate (n = 2). (B) Representative analysis of BHAV N protein expression in infected cell monolayers from the experiment shown in (A) . Cell lysates harvested at 5 d p.i. were analysed by western blotting using BHAV anti-N and anti-tubulin antibodies.

Journal: PLOS Pathogens

Article Title: Functional divergence of the Bhanja virus NSs protein modulates interferon antagonism and viral fitness across divergent lineages

doi: 10.1371/journal.ppat.1014645

Figure Lengend Snippet: (A) Replication of wtIG690 in a panel of vertebrate cell lines derived from different host species and tissues. Cell monolayers were infected with wtIG690 at an MOI of 0.01 FFU/cell (light blue) or 0.1 FFU/cell (dark blue). At 5 d p.i., culture supernatants were harvested and viral titres determined by focus-forming assay on Vero E6 cells. Viral titres are presented as Log 10 FFU/ml. M, monkey; Mo, mouse; V, vole; Ch, chicken; Qu, quail, Ho, horse; WBC, white blood cells; K, kidney; E, embryo; S, skin. Experiments were performed in duplicate (n = 2). (B) Representative analysis of BHAV N protein expression in infected cell monolayers from the experiment shown in (A) . Cell lysates harvested at 5 d p.i. were analysed by western blotting using BHAV anti-N and anti-tubulin antibodies.

Techniques: Derivative Assay, Infection, Focus Forming Assay, Analysis, Expressing, Western Blot

(A) Heat map showing pairwise amino acid identity (%) between the NSs proteins of five BHAV isolates. The following accession numbers corresponding to BHAV NSs proteins were used: IG690 ( AGK25093 ); ibAr2709 ( YP_009141016 ); R1819 ( AGC60109 ); R1329 ( PZ464570 ); R1336 ( PZ464571 ). (B) Schematic representation of recombinant rIG690 viruses expressing heterologous c-terminal V5-tagged NSs proteins derived from differing BHAV isolates, generating rIG690NSsV5 (orange), ribAr2709NSsV5 (yellow), rR1819NSsV5 (purple) and rR1329NSsV5 (blue). The NSs-deletant control virus expressing mGreenLantern (mGL; green) is also shown. (C) Replication kinetics of recombinant BHAV expressing heterologous V5-tagged NSs proteins in A549 (top) and A549-VNPro (bottom) cells. Cell monolayers were infected at an MOI of 0.1 FFU/cell and culture supernatants harvested at the indicated times p.i. for titration by focus-forming assay on Vero E6 cells. Viral titres are presented as Log 10 FFU/ml. Data represent the mean ± SD from three biological replicates. (D) Representative analysis of viral protein expression during infection with recombinant BHAV expressing heterologous V5-tagged NSs proteins. Cell lysates collected from infected A549 and A549-VNPro monolayers (described in (C) ) at the indicated times p.i. were analysed by western blotting using anti-V5, BHAV anti-N and anti-tubulin antibodies.

Journal: PLOS Pathogens

Article Title: Functional divergence of the Bhanja virus NSs protein modulates interferon antagonism and viral fitness across divergent lineages

doi: 10.1371/journal.ppat.1014645

Figure Lengend Snippet: (A) Heat map showing pairwise amino acid identity (%) between the NSs proteins of five BHAV isolates. The following accession numbers corresponding to BHAV NSs proteins were used: IG690 ( AGK25093 ); ibAr2709 ( YP_009141016 ); R1819 ( AGC60109 ); R1329 ( PZ464570 ); R1336 ( PZ464571 ). (B) Schematic representation of recombinant rIG690 viruses expressing heterologous c-terminal V5-tagged NSs proteins derived from differing BHAV isolates, generating rIG690NSsV5 (orange), ribAr2709NSsV5 (yellow), rR1819NSsV5 (purple) and rR1329NSsV5 (blue). The NSs-deletant control virus expressing mGreenLantern (mGL; green) is also shown. (C) Replication kinetics of recombinant BHAV expressing heterologous V5-tagged NSs proteins in A549 (top) and A549-VNPro (bottom) cells. Cell monolayers were infected at an MOI of 0.1 FFU/cell and culture supernatants harvested at the indicated times p.i. for titration by focus-forming assay on Vero E6 cells. Viral titres are presented as Log 10 FFU/ml. Data represent the mean ± SD from three biological replicates. (D) Representative analysis of viral protein expression during infection with recombinant BHAV expressing heterologous V5-tagged NSs proteins. Cell lysates collected from infected A549 and A549-VNPro monolayers (described in (C) ) at the indicated times p.i. were analysed by western blotting using anti-V5, BHAV anti-N and anti-tubulin antibodies.

Techniques: Recombinant, Expressing, Derivative Assay, Control, Virus, Infection, Titration, Focus Forming Assay, Analysis, Western Blot

(A) Sensitivity of rIG690 to exogenous IFNβ treatment. A549-NPro cells were treated with increasing concentrations of IFNβ (0, 10, 100 or 1000 U) either 24 h or 2 h prior to infection, or 2 h or 24 h p.i. Cells were infected with rIG690 at an MOI of 0.5 FFU/cell and culture supernatants harvested at 48 h p.i. for titration by focus-forming assay on Vero E6 cells. Viral titres are presented as Log 10 FFU/ml. (B) Sensitivity of recombinant BHAV expressing heterologous V5-tagged NSs proteins to IFNβ treatment. A549-NPro cells were pre-treated with either 0 or 1000 U of IFNβ for 24 h prior to infection with recombinant viruses expressing different NSs proteins from the rIG690 backbone at an MOI of 0.5 FFU/cell. Recombinant viruses included rIG690NSsV5 (orange), ribAr2709NSsV5 (yellow), rR1819NSsV5 (purple) and rR1329NSsV5 (green). The NSs-deletant virus rIG690ΔNSs-mGL (mGL; light green) was included as a control lacking NSs expression, while rIG690 was included to assess the effect of V5-tagging on NSs function (light orange). Culture supernatants were harvested at 48 h p.i. and viral titres determined by focus-forming assay on Vero E6 cells. Viral titres are presented as Log 10 FFU/ml. (C,D) Biological interferon protection assay used to assess suppression of IFN production by recombinant BHAV expressing heterologous NSs proteins. A549 cells were infected at an MOI of 2 FFU/cell with the indicated recombinant viruses. At 24 h p.i., culture supernatants were harvested, UV-inactivated and serially diluted two-fold prior to treatment of A549-NPro cells. Treated monolayers were subsequently infected with EMCV at an MOI of 0.05 PFU/cell. (C) Representative image of assay readout Relative IFN units are expressed as 2N, where N corresponds to the final two-fold dilution that retained protection from EMCV-induced cytopathic effect. Data shown in (A) and (B) represent the mean ± SD from three biological replicates, while data shown in (C) and (D) represent the mean ± SD from two biological replicates.

Journal: PLOS Pathogens

Article Title: Functional divergence of the Bhanja virus NSs protein modulates interferon antagonism and viral fitness across divergent lineages

doi: 10.1371/journal.ppat.1014645

Figure Lengend Snippet: (A) Sensitivity of rIG690 to exogenous IFNβ treatment. A549-NPro cells were treated with increasing concentrations of IFNβ (0, 10, 100 or 1000 U) either 24 h or 2 h prior to infection, or 2 h or 24 h p.i. Cells were infected with rIG690 at an MOI of 0.5 FFU/cell and culture supernatants harvested at 48 h p.i. for titration by focus-forming assay on Vero E6 cells. Viral titres are presented as Log 10 FFU/ml. (B) Sensitivity of recombinant BHAV expressing heterologous V5-tagged NSs proteins to IFNβ treatment. A549-NPro cells were pre-treated with either 0 or 1000 U of IFNβ for 24 h prior to infection with recombinant viruses expressing different NSs proteins from the rIG690 backbone at an MOI of 0.5 FFU/cell. Recombinant viruses included rIG690NSsV5 (orange), ribAr2709NSsV5 (yellow), rR1819NSsV5 (purple) and rR1329NSsV5 (green). The NSs-deletant virus rIG690ΔNSs-mGL (mGL; light green) was included as a control lacking NSs expression, while rIG690 was included to assess the effect of V5-tagging on NSs function (light orange). Culture supernatants were harvested at 48 h p.i. and viral titres determined by focus-forming assay on Vero E6 cells. Viral titres are presented as Log 10 FFU/ml. (C,D) Biological interferon protection assay used to assess suppression of IFN production by recombinant BHAV expressing heterologous NSs proteins. A549 cells were infected at an MOI of 2 FFU/cell with the indicated recombinant viruses. At 24 h p.i., culture supernatants were harvested, UV-inactivated and serially diluted two-fold prior to treatment of A549-NPro cells. Treated monolayers were subsequently infected with EMCV at an MOI of 0.05 PFU/cell. (C) Representative image of assay readout Relative IFN units are expressed as 2N, where N corresponds to the final two-fold dilution that retained protection from EMCV-induced cytopathic effect. Data shown in (A) and (B) represent the mean ± SD from three biological replicates, while data shown in (C) and (D) represent the mean ± SD from two biological replicates.

Techniques: Sensitivity, Infection, Titration, Focus Forming Assay, Recombinant, Expressing, Virus, Control

Review

vero e6 tmprss2 (ATCC)

Structured Review

Images

1) Product Images from "Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses"

Related Articles

Expressing:

Cell Culture:

Transfection:

Protein Purification:

SPR Assay:

Modification:

Similar Products

Image Search Results